Establishing comprehensive quaternary structural proteomes from genome sequence.

ABSTRACT: A critical body of knowledge has developed through advances in protein microscopy, protein-fold modeling, structural biology software, availability of sequenced bacterial genomes, large-scale mutation databases, and genome-scale models. Based on these recent advances, we develop a computational platform that; i) computes the oligomeric structural proteome encoded by an organism's genome; ii) maps multi-strain alleleomic variation, resulting in the structural proteome for a species; and iii) calculates the 3D orientation of proteins across subcellular compartments with angstrom-level precision. Using the platform, we; iv) compute the full quaternary E. coli K-12 MG1655 structural proteome; v) deploy structure-guided analyses to identify consequential mutations; and, in combination wi

SUBMITTER: Catoiu EA 

PROVIDER: S-EPMC10246253 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature