Correlated cryo-SEM and CryoNanoSIMS imaging of biological tissue.

ABSTRACT:

Background

The development of nanoscale secondary ion mass spectrometry (NanoSIMS) has revolutionized the study of biological tissues by enabling, e.g., the visualization and quantification of metabolic processes at subcellular length scales. However, the associated sample preparation methods all result in some degree of tissue morphology distortion and loss of soluble compounds. To overcome these limitations an entirely cryogenic sample preparation and imaging workflow is required.

Results

Here, we report the development of a CryoNanoSIMS instrument that can perform isotope imaging of both positive and negative secondary ions from flat block-face surfaces of vitrified biological tissues with a mass- and image resolution comparable to that of a conventional NanoSIMS. This ca

SUBMITTER: Meibom A 

PROVIDER: S-EPMC10246362 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature