Role of high-temperature requirement serine protease A 2 in rheumatoid inflammation.

ABSTRACT:

Background

High-temperature requirement serine protease A 2 (HtrA2) is known to be involved in growth, unfolded protein response to stress, apoptosis, and autophagy. However, whether HtrA2 controls inflammation and immune response remains elusive.

Methods

Expression of HtrA2 in the synovial tissue of patients was examined using immunohistochemistry and immunofluorescence staining. Enzyme-linked immunosorbent assay was used to determine the concentrations of HtrA2, interleukin-6 (IL-6), interleukin-8 (IL-8), chemokine (C-C motif) ligand 2 (CCL2), and tumor necrosis factor α (TNFα). Synoviocyte survival was assessed by MTT assay. For the downregulation of HtrA2 transcripts, cells were transfected with HtrA2 siRNA.

Results

We found that the concentration of HtrA2 was ele

SUBMITTER: Jeong GH 

PROVIDER: S-EPMC10246393 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature