Using light scattering to assess how phospholipid-protein interactions affect complex I functionality in liposomes.

ABSTRACT: Complex I is an essential membrane protein in respiration, oxidising NADH and reducing ubiquinone to contribute to the proton-motive force that powers ATP synthesis. Liposomes provide an attractive platform to investigate complex I in a phospholipid membrane with the native hydrophobic ubiquinone substrate and proton transport across the membrane, but without convoluting contributions from other proteins present in the native mitochondrial inner membrane. Here, we use dynamic and electrophoretic light scattering techniques (DLS and ELS) to show how physical parameters, in particular the zeta potential (ζ-potential), correlate strongly with the biochemical functionality of complex I-containing proteoliposomes. We find that cardiolipin plays a crucial role in the reconstitution and fu

SUBMITTER: Eisermann J 

PROVIDER: S-EPMC10246558 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature