Genomic Characteristics of a Multidrug-Resistant ST648 Escherichia coli Isolate Co-Carrying bla_KPC-2 and bla_CTX-M-15 Genes Recovered from a Respiratory Infection in China.

ABSTRACT:

Background

The transmission of carbapenem-resistant Enterobacterales pose a significant threat to global public health, which weakens the effectiveness of most antimicrobial agents. The aim of this study is to present the genomic characteristics of a multidrug-resistant Escherichia coli, which contains both bla_KPC-2 and bla_CTX-M-15 genes, discovered from a respiratory infection in China.

Methods

The antimicrobial susceptibility of E. coli isolate 488 was measured by using the broth microdilution method. The Oxford Nanopore MinION and Illumina NovaSeq 6000 platforms were applied to determine the whole-genome sequence of this isolate. De novo assembly of short Illumina reads and long MinION reads were performed by Unicycler. In

SUBMITTER: He X 

PROVIDER: S-EPMC10246572 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature