Evaluation of the Binding Preference of Microtubes for Nanoproteomics Sample Preparation.

ABSTRACT: Nonspecific binding between the protein and the container is an often-neglected cause of sample loss in large-scale proteomics sample preparation. In nanoproteomics, due to the small sample size, this absorption loss is no longer negligible, and researchers often adopt low binding plasticware to minimize the sample loss. However, there has been little discussion in the scientific literature on the differences in microtube performance on reducing protein/peptide binding. Therefore, the exact impact of sample loss during the sample preparation is not well understood. Here, we investigated the protein/peptide loss during the nanoproteomics experiment process. Our results showed that there are significant differences in nonspecific binding among the tested microtubes, with a protein recovery r

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC10246705 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature