Sortilin Inhibition Protects Neurons From Degeneration in the Diabetic Retina.

ABSTRACT:

Purpose

To investigate the level and localization of the multifunctional receptor sortilin in the diabetic retina, as well as the effect of sortilin inhibition on retinal neurodegeneration in experimental diabetes.

Methods

The localization of sortilin and colocalization with the p75 neurotrophin receptor (p75NTR) and Müller cell (MC) markers were determined using immunofluorescence on retinal sections from human patients with diabetes and streptozotocin-induced diabetic C57BL/6J male mice. In the diabetic mice, levels were further quantified using Western blot and quantitative PCR. Therapeutic studies were performed on diabetic mice using intravitreally injected anti-sortilin antibodies. Neuroprotection was evaluated in vivo by optical coherence tomography and by quantificat

SUBMITTER: Jakobsen TS 

PROVIDER: S-EPMC10246757 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature