3D deconvolution of human skin immune architecture with Multiplex Annotated Tissue Imaging System.

ABSTRACT: Routine clinical assays, such as conventional immunohistochemistry, often fail to resolve the regional heterogeneity of complex inflammatory skin conditions. We introduce MANTIS (Multiplex Annotated Tissue Imaging System), a flexible analytic pipeline compatible with routine practice, specifically designed for spatially resolved immune phenotyping of the skin in experimental or clinical samples. On the basis of phenotype attribution matrices coupled to α-shape algorithms, MANTIS projects a representative digital immune landscape while enabling automated detection of major inflammatory clusters and concomitant single-cell data quantification of biomarkers. We observed that severe pathological lesions from systemic lupus erythematosus, Kawasaki syndrome, or COVID-19-associated skin manifesta

SUBMITTER: Scholaert M 

PROVIDER: S-EPMC10246893 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature