Antibody- and aptamer-free SERS substrate for ultrasensitive and anti-interference detection of SARS-CoV-2 spike protein in untreated saliva

ABSTRACT: Sensitive and anti-interference detection of targeted signal(s) in body fluids is one of the paramount tasks in biosensing. Overcoming the complication and high cost of antibody/aptamer-modification, surface-enhanced Raman spectroscopy (SERS) based on antibody/aptamer-free (AAF) substrates has shown great promise, yet with rather limited detection sensitivity. Herein, we report ultrasensitive and anti-interference detection of SARS-CoV-2 spike protein in untreated saliva by an AAF SERS substrate, applying the evanescent field induced by the high-order waveguide modes of well-defined nanorods for SERS for the first time. A detection limit of 3.6 × 10−17 M and 1.6 × 10−16 M are obtained in phosphate buffered saline and untreated saliva, respectively; the detection limits are three orders of

SUBMITTER: Zhang H 

PROVIDER: S-EPMC10247595 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature