Comprehensive genetic analyses of childhood acute leukemia in Iraq using next-generation sequencing.

ABSTRACT:

Background

Molecular analyses in hematological malignancies provide insights about genetic makeup. Probable etiological factors in leukemogenesis could also be disclosed. Since genetic analyses are still primitive in Iraq, a country of repeated wars, we conceived of performing next-generation sequencing (NGS), to disclose the genomic landscape of acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML) among a cohort of Iraqi children.

Methods

Dried blood samples were collected from Iraqi children with ALL (n=55), or AML (n=11), and transferred to Japan where NGS was done. Whole-exome, whole-genome, and targeted gene sequencings were performed.

Results

Somatic point mutations and the copy number variations among Iraqi children with acute leukemia were compa

SUBMITTER: Al-Kzayer LFY 

PROVIDER: S-EPMC10248931 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature