Negative regulation of HBG1/2 expression through S6K by long noncoding RNA NR_120526.

ABSTRACT:

Background

High levels of fetal hemoglobin (HbF) may alleviate clinical symptoms in patients with β-thalassemia. A previous study showed that the long noncoding RNA NR_120526 (lncRNA NR_120526) might be involved in regulating HbF levels (HBG1/2 gene expression). However, the function and mechanism by which NR_120526 regulates HbF expression remains unknown. Here, we investigated the effect of NR_120526 on HbF and its mechanism so as to provide an experimental basis for treating patients with β-thalassemia.

Methods

Chromatin isolation by RNA purification-mass spectrometry (ChIRP-MS) assay, database query, and bioinformatics analysis were performed to explore the proteins that specifically bind to NR_120526 and their interactions. Chromatin immunoprecipitation fo

SUBMITTER: Jia W 

PROVIDER: S-EPMC10248940 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature