Dataset Information

MhCLC-c1, a Cl channel c homolog from Malus hupehensis, alleviates NaCl-induced cell death by inhibiting intracellular Cl^- accumulation.

ABSTRACT:

Background

Overaccumulation of chloride (Cl) when plants suffer NaCl causes cell damage and death, and is regulated by Cl^- channel protein (CLC). Apple roots are very sensitive to Cl^-, but information associated with CLC is limited in apple crop that widely cultivated in the world.

Results

We identified 9 CLCs from the apple genome and divided them into two subclasses. Among them, MdCLC-c1 promoter contained the largest number of cis-acting elements associated with NaCl stress, and only the MdCLC-c1, MdCLC-d, and MdCLC-g were predicted that may be Cl^- antiporters or channels. Expression analysis of MdCLCs homologs in the roots of Malus hupehensis showed that most of the MhCLCs expression were response to NaCl stress, especially MhCLC-c1 expression was upregulated continuously and rapidly expressed during NaCl treatment. Therefore, we isolated MhCLC-c1 and observed it was a plasma membrane-localized protein. The MhCLC-c1 suppression significantly increased sensitivity, reactive oxygen species content, and cell death of apple calli; while MhCLC-c1 overexpression decreased sensitivity, reactive oxygen species content, and cell death of apple calli and Arabidopsis by inhibiting intracellular Cl^- accumulation under NaCl stress.

Conclusions

The study selected and isolated a CLC-c gene MhCLC-c1 from Malus hupehensis based on identification of CLCs gene family in apple, and their homologs MhCLCs expression patterns during NaCl treatments, revealing that MhCLC-c1 alleviates NaCl-induced cell death by inhibiting intracellular Cl^- accumulation. Our findings confer the comprehensive and in-depth upstanding of the mechanism that plants resist salt stress, and might also confer genetic improvement of salt tolerance in horticultural crops and the development and utilization of saline-alkali land.

SUBMITTER: Song J

PROVIDER: S-EPMC10249150 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Publications

MhCLC-c1, a Cl channel c homolog from Malus hupehensis, alleviates NaCl-induced cell death by inhibiting intracellular Cl<sup>-</sup> accumulation.

Song Jianfei J Han Mengyuan M Zhu Xiaoyue X Li Huan H Ning Yuansheng Y Zhang Weiwei W Yang Hongqiang H

BMC plant biology 20230608 1

<h4>Background</h4>Overaccumulation of chloride (Cl) when plants suffer NaCl causes cell damage and death, and is regulated by Cl<sup>-</sup> channel protein (CLC). Apple roots are very sensitive to Cl<sup>-</sup>, but information associated with CLC is limited in apple crop that widely cultivated in the world.<h4>Results</h4>We identified 9 CLCs from the apple genome and divided them into two subclasses. Among them, MdCLC-c1 promoter contained the largest number of cis-acting elements associate ...[more]

PMID: 37286968

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Dataset Information

MhCLC-c1, a Cl channel c homolog from Malus hupehensis, alleviates NaCl-induced cell death by inhibiting intracellular Cl^- accumulation.

Background

Results

Conclusions

Publications

MhCLC-c1, a Cl channel c homolog from Malus hupehensis, alleviates NaCl-induced cell death by inhibiting intracellular Cl<sup>-</sup> accumulation.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

MhCLC-c1, a Cl channel c homolog from Malus hupehensis, alleviates NaCl-induced cell death by inhibiting intracellular Cl- accumulation.

Background

Results

Conclusions

Publications

MhCLC-c1, a Cl channel c homolog from Malus hupehensis, alleviates NaCl-induced cell death by inhibiting intracellular Cl<sup>-</sup> accumulation.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

MhCLC-c1, a Cl channel c homolog from Malus hupehensis, alleviates NaCl-induced cell death by inhibiting intracellular Cl^- accumulation.