Significant improvement of bone marrow-derived MSC expansion from MDS patients by defined xeno-free medium.

ABSTRACT:

Background

Robust and reliable in vitro and in vivo models of primary cells are necessary to study the pathomechanisms of Myelodysplastic Neoplasms (MDS) and identify novel therapeutic strategies. MDS-derived hematopoietic stem and progenitor cells (HSPCs) are reliant on the support of bone marrow (BM) derived mesenchymal stroma cells (MSCs). Therefore, isolation and expansion of MCSs are essential for successfully modeling this disease. For the clinical use of healthy MSCs isolated from human BM, umbilical cord blood or adipose tissue, several studies showed that xeno-free (XF) culture conditions resulted in superior growth kinetics compared to MSCs cultured in the presence of fetal bovine serum (FBS). In this present study, we investigate, whether the replacement of a commerciall

SUBMITTER: Altrock E 

PROVIDER: S-EPMC10249283 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature