Cholic and deoxycholic acids induce mitochondrial dysfunction, impaired biogenesis and autophagic flux in skeletal muscle cells.

ABSTRACT:

Background

Skeletal muscle is sensitive to bile acids (BA) because it expresses the TGR5 receptor for BA. Cholic (CA) and deoxycholic (DCA) acids induce a sarcopenia-like phenotype through TGR5-dependent mechanisms. Besides, a mouse model of cholestasis-induced sarcopenia was characterised by increased levels of serum BA and muscle weakness, alterations that are dependent on TGR5 expression. Mitochondrial alterations, such as decreased mitochondrial potential and oxygen consumption rate (OCR), increased mitochondrial reactive oxygen species (mtROS) and unbalanced biogenesis and mitophagy, have not been studied in BA-induced sarcopenia.

Methods

We evaluated the effects of DCA and CA on mitochondrial alterations in C₂C₁₂ myotubes and a mouse model of chol

SUBMITTER: Abrigo J 

PROVIDER: S-EPMC10249330 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature