Leveraging a Structural Blueprint to Rationally Engineer the Rieske Oxygenase TsaM.

ABSTRACT: Rieske nonheme iron oxygenases use two metallocenters, a Rieske-type [2Fe-2S] cluster and a mononuclear iron center, to catalyze oxidation reactions on a broad range of substrates. These enzymes are widely used by microorganisms to degrade environmental pollutants and to build complexity in a myriad of biosynthetic pathways that are industrially interesting. However, despite the value of this chemistry, there is a dearth of understanding regarding the structure-function relationships in this enzyme class, which limits our ability to rationally redesign, optimize, and ultimately exploit the chemistry of these enzymes. Therefore, in this work, by leveraging a combination of available structural information and state-of-the-art protein modeling tools, we show that three "hotspot" regions can

SUBMITTER: Tian J 

PROVIDER: S-EPMC10249351 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature