Evaluation of Per- and Polyfluoroalkyl Substances (PFAS) <i>In Vitro</i> Toxicity Testing for Developmental Neurotoxicity.

ABSTRACT: Per- and polyfluoroalkyl substances (PFAS) are a diverse set of commercial chemicals widely detected in humans and the environment. However, only a limited number of PFAS are associated with epidemiological or experimental data for hazard identification. To provide developmental neurotoxicity (DNT) hazard information, the work herein employed DNT new approach methods (NAMs) to generate in vitro screening data for a set of 160 PFAS. The DNT NAMs battery was comprised of the microelectrode array neuronal network formation assay (NFA) and high-content imaging (HCI) assays to evaluate proliferation, apoptosis, and neurite outgrowth. The majority of PFAS (118/160) were inactive or equivocal in the DNT NAMs, leaving 42 active PFAS that decreased measures of neural network connectivity and

SUBMITTER: Carstens KE 

PROVIDER: S-EPMC10249374 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature