Single-Shot Quantitative Polarization Imaging of Complex Birefringent Structure Dynamics.

ABSTRACT: Polarization light microscopes are powerful tools for probing molecular order and orientation in birefringent materials. While a number of polarization microscopy techniques are available to access steady-state properties of birefringent samples, quantitative measurements of the molecular orientation dynamics on the millisecond time scale have remained a challenge. We propose polarized shearing interference microscopy (PSIM), a single-shot quantitative polarization imaging method, for extracting the retardance and orientation angle of the laser beam transmitting through optically anisotropic specimens with complex structures. The measurement accuracy and imaging performance of PSIM are validated by imaging a birefringent resolution target and a bovine tendon specimen. We demonstrate that P

SUBMITTER: Ge B 

PROVIDER: S-EPMC10249439 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature