A single-cell RNA-seq analysis unravels the heterogeneity of primary cultured human corneal endothelial cells.

ABSTRACT: The cornea is a transparent and avascular tissue located in front of the eye. Its inner surface is lined by a monolayer of corneal endothelial cells (CECs), which maintain the cornea transparency. CECs remain arrested in a non-proliferative state and damage to these cells can compromise their function leading to corneal opacity. The primary culture of donor-derived CECs is a promising cell therapy. It confers the potential to treat multiple patients from a single donor, alleviating the global donor shortage. Nevertheless, this approach has limitations preventing its adoption, particularly culture protocols allow limited expansion of CECs and there is a lack of clear parameters to identify therapy-grade CECs. To address this limitation, a better understanding of the molecular changes arisin

SUBMITTER: Catala P 

PROVIDER: S-EPMC10249941 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature