Cerebroprotective Role of N⁶-Methyladenosine Demethylase FTO (Fat Mass and Obesity-Associated Protein) After Experimental Stroke.

ABSTRACT:

Background

FTO (fat mass and obesity-associated protein) demethylates N⁶-methyladenosine (m⁶A), which is a critical epitranscriptomic regulator of neuronal function. We previously reported that ischemic stroke induces m⁶A hypermethylation with a simultaneous decrease in FTO expression in neurons. Currently, we evaluated the functional significance of restoring FTO with an adeno-associated virus 9, and thus reducing m⁶A methylation in poststroke brain damage.

Methods

Adult male and female C57BL/6J mice were injected with FTO adeno-associated virus 9 (intracerebral) at 21 days prior to inducing transient middle cerebral artery occlusion. Poststroke brain damage (infarction, atrophy, and white matter integrity) and neurobehavio

SUBMITTER: Chokkalla AK 

PROVIDER: S-EPMC10250008 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature