COVID-19 monitoring with sparse sampling of sewered and non-sewered wastewater in urban and rural communities.

ABSTRACT: Equitable SARS-CoV-2 surveillance in low-resource communities lacking centralized sewers is critical as wastewater-based epidemiology (WBE) progresses. However, large-scale studies on SARS-CoV-2 detection in wastewater from low-and middle-income countries is limited because of economic and technical reasons. In this study, wastewater samples were collected twice a month from 186 urban and rural subdistricts in nine provinces of Thailand mostly having decentralized and non-sewered sanitation infrastructure and analyzed for SARS-CoV-2 RNA variants using allele-specific RT-qPCR. Wastewater SARS-CoV-2 RNA concentration was used to estimate the real-time incidence and time-varying effective reproduction number (R_e). Results showed an increase in SARS-CoV-2 RNA concentrations in waste

SUBMITTER: Wannigama DL 

PROVIDER: S-EPMC10250052 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature