Short tandem repeats are important contributors to silencer elements in T cells.

ABSTRACT: The action of cis-regulatory elements with either activation or repression functions underpins the precise regulation of gene expression during normal development and cell differentiation. Gene activation by the combined activities of promoters and distal enhancers has been extensively studied in normal and pathological contexts. In sharp contrast, gene repression by cis-acting silencers, defined as genetic elements that negatively regulate gene transcription in a position-independent fashion, is less well understood. Here, we repurpose the STARR-seq approach as a novel high-throughput reporter strategy to quantitatively assess silencer activity in mammals. We assessed silencer activity from DNase hypersensitive I sites in a mouse T cell line. Identified silencers were associated with eith

SUBMITTER: Hussain S 

PROVIDER: S-EPMC10250210 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature