Discovery and characterization of novel Cre-type tyrosine site-specific recombinases for advanced genome engineering.

ABSTRACT: Tyrosine-type site-specific recombinases (Y-SSRs) are versatile tools for genome engineering due to their ability to mediate excision, integration, inversion and exchange of genomic DNA with single nucleotide precision. The ever-increasing need for sophisticated genome engineering is driving efforts to identify novel SSR systems with intrinsic properties more suitable for particular applications. In this work, we develop a systematic computational workflow for annotation of putative Y-SSR systems and apply this pipeline to identify and characterize eight new naturally occurring Cre-type SSR systems. We test their activity in bacterial and mammalian cells and establish selectivity profiles for the new and already established Cre-type SSRs with regard to their ability to mutually recombine t

SUBMITTER: Jelicic M 

PROVIDER: S-EPMC10250229 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature