THRONCAT: metabolic labeling of newly synthesized proteins using a bioorthogonal threonine analog.

ABSTRACT: Profiling the nascent cellular proteome and capturing early proteomic changes in response to external stimuli provides valuable insights into cellular physiology. Existing metabolic protein labeling approaches based on bioorthogonal methionine- or puromycin analogs allow for the selective visualization and enrichment of newly synthesized proteins. However, their applications are limited as they often require methionine-free conditions, auxotrophic cells and/or are toxic to cells. Here, we introduce THRONCAT, a threonine-derived non-canonical amino acid tagging method based on the bioorthogonal threonine analog β-ethynylserine (βES) that enables efficient labeling of the nascent proteome in complete growth media within minutes. We use THRONCAT for the visualization and enrichment of nascent

SUBMITTER: Ignacio BJ 

PROVIDER: S-EPMC10250548 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature