Allogenic multilayered fibroblast sheets promote anastomotic site healing in a rat model of esophageal reconstruction.

ABSTRACT:

Objective

Anastomotic leakage is a common and severe complication of esophageal reconstruction. Accordingly, there is a clinical need for novel methods to prevent it. We developed multilayered, growth factor-secreting fibroblast sheets that promote wound healing and angiogenesis. The present study aimed to assess the utility of allogenic multilayered fibroblast sheets in preventing esophageal anastomotic leakage in a rat model of esophageal reconstruction.

Methods

Allogenic multilayered fibroblast sheets prepared from oral mucosal tissues were implanted at esophageal anastomotic sites.

Results

The allogenic multilayered fibroblast sheet group had significantly higher burst pressure and collagen deposition compared to a control group five days postoperatively. The expr

SUBMITTER: Yamamoto N 

PROVIDER: S-EPMC10250989 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature