Dataset Information

Radial extracorporeal shock wave therapy alleviates acute inflammation of human primary tenocytes through the integrin-FAK-p38MAPK pathway.

ABSTRACT:

Objectives

To explore whether radial extracorporeal shock wave therapy (rESWT) can alleviate acute inflammation of human primary tenocytes by the integrin-focal adhesion kinase (FAK)-p38 mitogen-activated protein kinase (MAPK) pathway.

Methods

Western blotting was used to evaluate the changes in the integrin-FAK-p38MAPK signaling pathway mediated by rESWT using specific antibodies targeting the phosphorylation sites of intracellular signal pathway proteins.

Results

rESWT up-regulated FAK phosphorylation and down-regulated p38MAPK phosphorylation levels in a tumor necrosis factor (TNF)-α-induced acute inflammation model of human primary tenocytes. Pretreatment with an integrin inhibitor significantly reduced rESWT-mediated downregulation of p38MAPK phosphorylation and attenuated its reversal effect on the increased secretion of pro-inflammatory cytokines in TNF-α-induced human primary tenocytes.

Conclusions

Our results imply that rESWT may partially alleviate acute inflammation in human primary tenocytes through the integrin-FAK-p38MAPK pathway.

SUBMITTER: Ge R

PROVIDER: S-EPMC10251031 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Radial extracorporeal shock wave therapy alleviates acute inflammation of human primary tenocytes through the integrin-FAK-p38MAPK pathway.

Ge Ruidong R Ruan Bin B Chen Shengxuan S Bai Shuo S Gao Qi Q Dong Anran A

American journal of translational research 20230515 5

<h4>Objectives</h4>To explore whether radial extracorporeal shock wave therapy (rESWT) can alleviate acute inflammation of human primary tenocytes by the integrin-focal adhesion kinase (FAK)-p38 mitogen-activated protein kinase (MAPK) pathway.<h4>Methods</h4>Western blotting was used to evaluate the changes in the integrin-FAK-p38MAPK signaling pathway mediated by rESWT using specific antibodies targeting the phosphorylation sites of intracellular signal pathway proteins.<h4>Results</h4>rESWT up ...[more]

PMID: 37303680

Similar Datasets

Project description:BackgroundConflicting reports in the literature have raised the question whether radial extracorporeal shock wave therapy (rESWT) devices and vibrating massage devices have similar energy signatures and, hence, cause similar bioeffects in treated tissues.Methods and findingsWe used laser fiber optic probe hydrophone (FOPH) measurements, high-speed imaging and x-ray film analysis to compare fundamental elements of the energy signatures of two rESWT devices (Swiss DolorClast; Electro Medical Systems, Nyon, Switzerland; D-Actor 200; Storz Medical, Tägerwillen, Switzerland) and a vibrating massage device (Vibracare; G5/General Physiotherapy, Inc., Earth City, MO, USA). To assert potential bioeffects of these treatment modalities we investigated the influence of rESWT and vibrating massage devices on locomotion ability of Caenorhabditis elegans (C. elegans) worms.ResultsFOPH measurements demonstrated that both rESWT devices generated acoustic waves with comparable pressure and energy flux density. Furthermore, both rESWT devices generated cavitation as evidenced by high-speed imaging and caused mechanical damage on the surface of x-ray film. The vibrating massage device did not show any of these characteristics. Moreover, locomotion ability of C. elegans was statistically significantly impaired after exposure to radial extracorporeal shock waves but was unaffected after exposure of worms to the vibrating massage device.ConclusionsThe results of the present study indicate that both energy signature and bioeffects of rESWT devices are fundamentally different from those of vibrating massage devices.Clinical relevancePrior ESWT studies have shown that tissues treated with sufficient quantities of acoustic sound waves undergo cavitation build-up, mechanotransduction, and ultimately, a biological alteration that "kick-starts" the healing response. Due to their different treatment indications and contra-indications rESWT devices cannot be equated to vibrating massage devices and should be used with due caution in clinical practice.

Dataset Information

Radial extracorporeal shock wave therapy alleviates acute inflammation of human primary tenocytes through the integrin-FAK-p38MAPK pathway.

Objectives

Methods

Results

Conclusions

Publications

Radial extracorporeal shock wave therapy alleviates acute inflammation of human primary tenocytes through the integrin-FAK-p38MAPK pathway.

Similar Datasets

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