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Radial extracorporeal shock wave therapy alleviates acute inflammation of human primary tenocytes through the integrin-FAK-p38MAPK pathway.


ABSTRACT:

Objectives

To explore whether radial extracorporeal shock wave therapy (rESWT) can alleviate acute inflammation of human primary tenocytes by the integrin-focal adhesion kinase (FAK)-p38 mitogen-activated protein kinase (MAPK) pathway.

Methods

Western blotting was used to evaluate the changes in the integrin-FAK-p38MAPK signaling pathway mediated by rESWT using specific antibodies targeting the phosphorylation sites of intracellular signal pathway proteins.

Results

rESWT up-regulated FAK phosphorylation and down-regulated p38MAPK phosphorylation levels in a tumor necrosis factor (TNF)-α-induced acute inflammation model of human primary tenocytes. Pretreatment with an integrin inhibitor significantly reduced rESWT-mediated downregulation of p38MAPK phosphorylation and attenuated its reversal effect on the increased secretion of pro-inflammatory cytokines in TNF-α-induced human primary tenocytes.

Conclusions

Our results imply that rESWT may partially alleviate acute inflammation in human primary tenocytes through the integrin-FAK-p38MAPK pathway.

SUBMITTER: Ge R 

PROVIDER: S-EPMC10251031 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Radial extracorporeal shock wave therapy alleviates acute inflammation of human primary tenocytes through the integrin-FAK-p38MAPK pathway.

Ge Ruidong R   Ruan Bin B   Chen Shengxuan S   Bai Shuo S   Gao Qi Q   Dong Anran A  

American journal of translational research 20230515 5


<h4>Objectives</h4>To explore whether radial extracorporeal shock wave therapy (rESWT) can alleviate acute inflammation of human primary tenocytes by the integrin-focal adhesion kinase (FAK)-p38 mitogen-activated protein kinase (MAPK) pathway.<h4>Methods</h4>Western blotting was used to evaluate the changes in the integrin-FAK-p38MAPK signaling pathway mediated by rESWT using specific antibodies targeting the phosphorylation sites of intracellular signal pathway proteins.<h4>Results</h4>rESWT up  ...[more]

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