IL-15-producing splenic B cells play pathogenic roles in the development of autoimmune hepatitis.

ABSTRACT:

Background & aims

B-cell depletion therapy with an anti-CD20 is an effective treatment strategy for patients with refractory autoimmune hepatitis (AIH). However, the mechanisms underlying B-cell action are unclear.

Methods

Herein, we used the adeno-associated virus IL-12 model, in which hepatic IL-12 expression triggers liver injuries characteristic of AIH. We also analysed the clinical samples of patients with AIH.

Results

B-cell depletion using anti-CD20 or splenectomy was found to improve liver functions and decrease the cytotoxic CD8⁺ T-cell (cytotoxic T lymphocyte [CTL]) count in the liver. This improvement was reversed by the adoptive transfer of splenic B cells derived from AAV IL-12-treated mice to splenectomised mice as it caused the hepatic CTL co

SUBMITTER: Fujimori S 

PROVIDER: S-EPMC10251155 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature