Evaluation of Murine Host Sex as a Biological Variable in Transplanted Human Intestinal Organoid Development.

ABSTRACT:

Background

Human intestinal organoids (HIOs), when transplanted into immunocompromised mice (tHIOs), demonstrate significant growth and maturation. While both male and female mice are reported to be viable hosts for these experiments, a direct comparison of sex-related differences in tHIO structure and development has not been performed.

Aims

We sought to identify host sex-related differences in tHIO engraftment, morphology, and epithelial and mesenchymal development.

Methods

HIOs were generated in vitro and transplanted beneath the kidney capsule of NSG male and female mice. tHIOs were harvested at 8-9 weeks. Anthropometric measurements were captured. tHIOs were divided in half and histology or RT-qPCR performed. Morphology was evaluated and epithelial architecture g

SUBMITTER: McNeill EP 

PROVIDER: S-EPMC10251489 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature