Mass spectrometry imaging as an emerging tool for studying metabolism in human brain organoids.

ABSTRACT: Human brain organoids are emerging models to study human brain development and pathology as they recapitulate the development and characteristics of major neural cell types, and enable manipulation through an in vitro system. Over the past decade, with the advent of spatial technologies, mass spectrometry imaging (MSI) has become a prominent tool for metabolic microscopy, providing label-free, non-targeted molecular and spatial distribution information of the metabolites within tissue, including lipids. This technology has never been used for studies of brain organoids and here, we set out to develop a standardized protocol for preparation and mass spectrometry imaging of human brain organoids. We present an optimized and validated sample preparation protocol, including sample fixat

SUBMITTER: Cappuccio G 

PROVIDER: S-EPMC10251497 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature