The Edge Effect in High-Throughput Proteomics: A Cautionary Tale.

ABSTRACT: In order for mass spectrometry to continue to grow as a platform for high-throughput clinical and translational research, careful consideration must be given to quality control by ensuring that the assay performs reproducibly and accurately and precisely. In particular, the throughput required for large cohort clinical validation in biomarker discovery and diagnostic screening has driven the growth of multiplexed targeted liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) assays paired with sample preparation and analysis in multiwell plates. However, large scale MS-based proteomics studies are often plagued by batch effects: sources of technical variation in the data, which can arise from a diverse array of sources such as sample preparation batches, different reagent lo

SUBMITTER: Maxwell CB 

PROVIDER: S-EPMC10251511 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature