Genome-wide DNA methylation profiling of CD4⁺ T lymphocytes identifies differentially methylated loci associated with adult primary refractory immune thrombocytopenia.

ABSTRACT:

Background

DNA methylation played a crucial role in the pathogenesis of immune thrombocytopenia (ITP). However, genome-wide DNA methylation analysis has not been applied thus far. The present study aimed to provide the first DNA methylation profiling for ITP.

Methods

Peripheral blood CD4⁺ T lymphocytes samples were collected from 4 primary refractory ITP cases and 4 age-matched healthy controls, and DNA methylome profiling was performed using Infinium MethylationEPIC BeadChip. Differentially methylated CpG sites were further validated in another independent cohort of 10 ITP patients and 10 healthy controls using qRT-PCR.

Results

The DNA methylome profiling identified a total of 260 differentially methylated CpG sites mapping to 72 hypermethylated and 64 hyp

SUBMITTER: Du H 

PROVIDER: S-EPMC10251572 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature