Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Author Correction: INSERT-seq enables high-resolution mapping of genomically integrated DNA using Nanopore sequencing.

ABSTRACT:

SUBMITTER: Ivancic D

PROVIDER: S-EPMC10251592 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Author Correction: INSERT-seq enables high-resolution mapping of genomically integrated DNA using Nanopore sequencing.

Ivančić Dimitrije D Mir-Pedrol Júlia J Jaraba-Wallace Jessica J Rafel Núria N Sanchez-Mejias Avencia A Güell Marc M

Genome biology 20230609 1

PMID: 37296438

Similar Datasets

INSERT-seq enables high-resolution mapping of genomically integrated DNA using Nanopore sequencing.

Project description:Comprehensive characterisation of genome engineering technologies is relevant for their development and safe use in human gene therapy. Short-read based methods can overlook insertion events in repetitive regions. We develop INSERT-seq, a method that combines targeted amplification of integrated DNA, UMI-based correction of PCR bias and Oxford Nanopore long-read sequencing for robust analysis of DNA integration. The experimental pipeline improves the number of mappable insertions at repetitive regions by 4.8-7.3% and larger repeats are processed with a computational peak calling pipeline. INSERT-seq is a simple, cheap and robust method to quantitatively characterise DNA integration in diverse ex vivo and in vivo samples.

| S-EPMC9594898 | biostudies-literature

Author Correction: Hallmarks of a genomically distinct subclass of head and neck cancer.

Project description: Not available

| S-EPMC11833038 | biostudies-literature

Author Correction: Accurate detection of circulating tumor DNA using nanopore consensus sequencing.

Project description: Not available

| S-EPMC10247772 | biostudies-literature

Author Correction: SMURF-seq: efficient copy number profiling on long-read sequencers.

Project description:An amendment to this paper has been published and can be accessed via the original article.

| S-EPMC7447571 | biostudies-literature

Author Correction: Rapid de novo assembly of the European eel genome from nanopore sequencing reads.

Project description:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.

| S-EPMC6529464 | biostudies-literature

Integrated nanopore sensing platform with sub-microsecond temporal resolution.

Project description:Nanopore sensors have attracted considerable interest for high-throughput sensing of individual nucleic acids and proteins without the need for chemical labels or complex optics. A prevailing problem in nanopore applications is that the transport kinetics of single biomolecules are often faster than the measurement time resolution. Methods to slow down biomolecular transport can be troublesome and are at odds with the natural goal of high-throughput sensing. Here we introduce a low-noise measurement platform that integrates a complementary metal-oxide semiconductor (CMOS) preamplifier with solid-state nanopores in thin silicon nitride membranes. With this platform we achieved a signal-to-noise ratio exceeding five at a bandwidth of 1 MHz, which to our knowledge is the highest bandwidth nanopore recording to date. We demonstrate transient signals as brief as 1 ?s from short DNA molecules as well as current signatures during molecular passage events that shed light on submolecular DNA configurations in small nanopores.

| S-EPMC3648419 | biostudies-literature

Author Correction: Detection of aberrant splicing events in RNA-seq data using FRASER.

Project description: Not available

| S-EPMC9203570 | biostudies-literature

Author Correction: Mapping effective connectivity of human amygdala subdivisions with intracranial stimulation.

Project description: Not available

| S-EPMC9515150 | biostudies-literature

Author Correction: Geostatistical spatial projection of geophysical parameters for practical aquifer mapping.

Project description: Not available

| S-EPMC9669009 | biostudies-literature

Author Correction: Systematic protein-protein interaction mapping for clinically relevant human GPCRs.

Project description: Not available