Design of an Arabidopsis thaliana reporter line to detect heat-sensing and signaling mutants.

ABSTRACT:

Background

Global warming is a major challenge for plant survival and growth. Understanding the molecular mechanisms by which higher plants sense and adapt to upsurges in the ambient temperature is essential for developing strategies to enhance plant tolerance to heat stress. Here, we designed a heat-responsive Arabidopsis thaliana reporter line that allows an in-depth investigation of the mechanisms underlying the accumulation of protective heat-shock proteins (HSPs) in response to high temperature.

Methods

A transgenic Arabidopsis thaliana reporter line named "Heat-Inducible Bioluminescence And Toxicity" (HIBAT) was designed to express from a conditional heat-inducible promoter, a fusion gene encoding for nanoluciferase and D-amino acid oxidase, whose expression is toxic i

SUBMITTER: Guihur A 

PROVIDER: S-EPMC10251684 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature