Absence of changes in the milk microbiota during Escherichia coli endotoxin induced experimental bovine mastitis.

ABSTRACT: Changes in the milk microbiota during the course of mastitis are due to the nature of a sporadic occurring disease difficult to study. In this study we experimentally induced mastitis by infusion of Escherichia coli endotoxins in one udder quarter each of nine healthy lactating dairy cows and assessed the bacteriological dynamics and the milk microbiota at four time points before and eight time points after infusion. As control, saline was infused in one udder quarter each of additionally nine healthy cows that followed the same sampling protocol. The milk microbiota was assessed by sequencing of the 16 S rRNA gene and a range of positive and negative controls were included for methodological evaluation. Two different data filtration models were used to identify and cure data from contamin

SUBMITTER: Dahlberg J 

PROVIDER: S-EPMC10251687 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature