Recombinant Dense Granule Protein (NcGRA4) Is a Novel Serological Marker for Neospora caninum Infection in Goats

ABSTRACT:

Simple Summary

Neosporosis caused by Neospora caninum is now recognised globally as one of the major causes of productivity losses in small and large ruminants. Its diagnosis is primarily based on serological techniques, and various serological tests have been developed utilising native-soluble antigens or live tachyzoites. However, these tests frequently cross-react with proteins from other protozoa. To overcome this problem, we produced a recombinant protein from a synthetic NcGRA4 gene and examined the ability to detect N. caninum infection in field samples using indirect enzyme-linked immunosorbent assay (iELISA). The technique demonstrated good specificity and sensitivity, and its use on serum samples from the field revealed that it may be used to effectively diagnose neospor

SUBMITTER: Udonsom R 

PROVIDER: S-EPMC10251823 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature