DNA Polymerases for Whole Genome Amplification: Considerations and Future Directions.

ABSTRACT: In the same way that specialized DNA polymerases (DNAPs) replicate cellular and viral genomes, only a handful of dedicated proteins from various natural origins as well as engineered versions are appropriate for competent exponential amplification of whole genomes and metagenomes (WGA). Different applications have led to the development of diverse protocols, based on various DNAPs. Isothermal WGA is currently widely used due to the high performance of Φ29 DNA polymerase, but PCR-based methods are also available and can provide competent amplification of certain samples. Replication fidelity and processivity must be considered when selecting a suitable enzyme for WGA. However, other properties, such as thermostability, capacity to couple replication, and double helix unwinding, or the abili

SUBMITTER: Ordonez CD 

PROVIDER: S-EPMC10253169 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature