Transcription Factor VvDREB2A from Vitis vinifera Improves Cold Tolerance.

ABSTRACT: Low temperatures restrict the growth of the grapevine industry. The DREB transcription factors are involved in the abiotic stress response. Here, we isolated the VvDREB2A gene from Vitis vinifera cultivar 'Zuoyouhong' tissue culture seedlings. The full-length VvDREB2A cDNA was 1068 bp, encoding 355 amino acids, which contained an AP2 conserved domain belonging to the AP2 family. Using transient expression in leaves of tobacco, VvDREB2A was localized to the nucleus, and it potentiated transcriptional activity in yeasts. Expression analysis revealed that VvDREB2A was expressed in various grapevine tissues, with the highest expression in leaves. VvDREB2A was induced by cold and the stress-signaling molecules H₂S, nitric oxide, and abscisic acid. F

SUBMITTER: Hou L 

PROVIDER: S-EPMC10253714 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature