Dataset Information

Conjugated Dienoic Acid Peroxides as Substrates in Chaetopterus Bioluminescence System.

ABSTRACT: Biochemistry of bioluminescence of the marine parchment tubeworm Chaetopterus has been in research focus for over a century; however, the results obtained by various groups contradict each other. Here, we report the isolation and structural elucidation of three compounds from Chaetomorpha linum algae, which demonstrate bioluminescence activity with Chaetopterus luciferase in the presence of Fe²⁺ ions. These compounds are derivatives of polyunsaturated fatty acid peroxides. We have also obtained their structural analogues and demonstrated their activity in the bioluminescence reaction, thus confirming the broad substrate specificity of the luciferase.

SUBMITTER: Zagitova RI

PROVIDER: S-EPMC10253912 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

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Conjugated Dienoic Acid Peroxides as Substrates in Chaetopterus Bioluminescence System.

Zagitova Renata I RI Purtov Konstantin V KV Shcheglov Aleksandr S AS Mineev Konstantin S KS Dubinnyi Maxim A MA Myasnyanko Ivan N IN Belozerova Olga A OA Pakhomova Vera G VG Petushkov Valentin N VN Rodionova Natalia S NS Lushpa Vladislav A VA Guglya Elena B EB Kovalchuk Sergey S Kozhemyako Valeri B VB Mirza Jeremy D JD Oliveira Anderson G AG Yampolsky Ilia V IV Kaskova Zinaida M ZM Tsarkova Aleksandra S AS

International journal of molecular sciences 20230530 11

Biochemistry of bioluminescence of the marine parchment tubeworm Chaetopterus has been in research focus for over a century; however, the results obtained by various groups contradict each other. Here, we report the isolation and structural elucidation of three compounds from Chaetomorpha linum algae, which demonstrate bioluminescence activity with Chaetopterus luciferase in the presence of Fe2+ ions. These compounds are derivatives of polyunsaturated fatty acid p ...[more]

PMID: 37298416

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Project description:Conjugated linoleic acids (CLAs) and conjugated linolenic acids (CLNAs) have gained significant attention due to their anticarcinogenic and lipid/energy metabolism-modulatory effects. However, their concentration in foodstuffs is insufficient for any therapeutic application to be implemented. From a biotechnological standpoint, microbial production of these conjugated fatty acids (CFAs) has been explored as an alternative, and strains of the genera Propionibacterium, Lactobacillus, and Bifidobacterium have shown promising producing capacities. Current screening research works are generally based on direct analytical determination of production capacity (e.g., trial and error), representing an important bottleneck in these studies. This review aims to summarize the available information regarding identified genes and proteins involved in CLA/CLNA production by these groups of bacteria and, consequently, the possible enzymatic reactions behind such metabolic processes. Linoleate isomerase (LAI) was the first enzyme to be described to be involved in the microbiological transformation of linoleic acids (LAs) and linolenic acids (LNAs) into CFA isomers. Thus, the availability of lai gene sequences has allowed the development of genetic screening tools. Nevertheless, several studies have reported that LAIs have significant homology with myosin-cross-reactive antigen (MCRA) proteins, which are involved in the synthesis of hydroxy fatty acids, as shown by hydratase activity. Furthermore, it has been suggested that CLA and/or CLNA production results from a stress response performed by the activation of more than one gene in a multiple-step reaction. Studies on CFA biochemical pathways are essential to understand and characterize the metabolic mechanism behind this process, unraveling all the gene products that may be involved. As some of these bacteria have shown modulation of lipid metabolism in vivo, further research to be focused on this topic may help us to understand the role of the gut microbiota in human health.

Dataset Information

Conjugated Dienoic Acid Peroxides as Substrates in Chaetopterus Bioluminescence System.

Publications

Conjugated Dienoic Acid Peroxides as Substrates in <i>Chaetopterus</i> Bioluminescence System.

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