A Triazaspirane Derivative Inhibits Migration and Invasion in PC3 Prostate Cancer Cells.

ABSTRACT: Cancer is a serious health problem due to the complexity of establishing an effective treatment. The purpose of this work was to evaluate the activity of a triazaspirane as a migration and invasion inhibitor in PC3 prostatic tumor cells through a possible negative regulation of the FAK/Src signal transduction pathway and decreased secretion of metalloproteinases 2 and 9. Molecular docking analysis was performed using Moe 2008.10 software. Migration (wound-healing assay) and invasion (Boyden chamber assay) assays were performed. In addition, the Western blot technique was used to quantify protein expression, and the zymography technique was used to observe the secretion of metalloproteinases. Molecular docking showed interactions in regions of interest of the FAK and Src proteins. Moreover,

SUBMITTER: Vasconcelos-Ulloa JJ 

PROVIDER: S-EPMC10254162 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature