Cell surface marker-based capture of neoantigen-reactive CD8⁺ T-cell receptors from metastatic tumor digests.

ABSTRACT:

Background

Cellular immunotherapies using autologous tumor-infiltrating lymphocytes (TIL) can induce durable regression of epithelial cancers in selected patients with treatment-refractory metastatic disease. As the genetic engineering of T cells with tumor-reactive T-cell receptors (TCRs) comes to the forefront of clinical investigation, the rapid, scalable, and cost-effective detection of patient-specific neoantigen-reactive TIL remains a top priority.

Methods

We analyzed the single-cell transcriptomic states of 31 neoantigen-specific T-cell clonotypes to identify cell surface dysfunction markers that best identified the metastatic transcriptional states enriched with antitumor TIL. We developed an efficient method to capture neoantigen-reactive TCRs directly from resected

SUBMITTER: Chatani PD 

PROVIDER: S-EPMC10255266 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature