Multiplexable and Biocomputational Virus Detection by CRISPR-Cas9-Mediated Strand Displacement.

ABSTRACT: Recurrent disease outbreaks caused by different viruses, including the novel respiratory virus SARS-CoV-2, are challenging our society at a global scale; so versatile virus detection methods would enable a calculated and faster response. Here, we present a novel nucleic acid detection strategy based on CRISPR-Cas9, whose mode of action relies on strand displacement rather than on collateral catalysis, using the Streptococcus pyogenes Cas9 nuclease. Given a preamplification process, a suitable molecular beacon interacts with the ternary CRISPR complex upon targeting to produce a fluorescent signal. We show that SARS-CoV-2 DNA amplicons generated from patient samples can be detected with CRISPR-Cas9. We also show that CRISPR-Cas9 allows the simultaneous detection of different DNA ampl

SUBMITTER: Marquez-Costa R 

PROVIDER: S-EPMC10255568 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature