Histopathologic and transcriptomic phenotypes of a conditional RANKL transgenic mouse thymus.

ABSTRACT: Although conventional knockout and transgenic mouse models have significantly advanced our understanding of Receptor Activator of NF-κB Ligand (RANKL) signaling in intra-thymic crosstalk that establishes self-tolerance and later stages of lymphopoiesis, the unique advantages of conditional mouse transgenesis have yet to be explored. A main advantage of conditional transgenesis is the ability to express a transgene in a spatiotemporal restricted manner, enabling the induction (or de-induction) of transgene expression during predetermined stages of embryogenesis or during defined postnatal developmental or physiological states, such as puberty, adulthood, and pregnancy. Here, we describe the K5: RANKL bigenic mouse, in which transgene derived RANKL expression is induced by doxycycline and ta

SUBMITTER: Szwarc MM 

PROVIDER: S-EPMC10255830 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature