Experimental Analysis of Tear Fluid and Its Processing for the Diagnosis of Multiple Sclerosis.

ABSTRACT: A pilot analysis of the tear fluid of patients with multiple sclerosis (MS) collected by glass microcapillary was performed using various experimental methods: liquid chromatography-mass spectrometry, Raman spectroscopy, infrared spectroscopy, and atomic-force microscopy. Infrared spectroscopy found no significant difference between the tear fluid of MS patients and the control spectra; all three significant peaks were located at around the same positions. Raman analysis showed differences between the spectra of the tear fluid of MS patients and the spectra of healthy subjects, which indicated a decrease in tryptophan and phenylalanine content and changes in the relative contributions of the secondary structures of the polypeptide chains of tear proteins. Atomic-force microscopy exhibited

SUBMITTER: Tomeckova V 

PROVIDER: S-EPMC10256036 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature