Neural network and kinetic modelling of human genome replication reveal replication origin locations and strengths.

ABSTRACT: In human and other metazoans, the determinants of replication origin location and strength are still elusive. Origins are licensed in G1 phase and fired in S phase of the cell cycle, respectively. It is debated which of these two temporally separate steps determines origin efficiency. Experiments can independently profile mean replication timing (MRT) and replication fork directionality (RFD) genome-wide. Such profiles contain information on multiple origins' properties and on fork speed. Due to possible origin inactivation by passive replication, however, observed and intrinsic origin efficiencies can markedly differ. Thus, there is a need for methods to infer intrinsic from observed origin efficiency, which is context-dependent. Here, we show that MRT and RFD data are highly consistent w

SUBMITTER: Arbona JM 

PROVIDER: S-EPMC10256156 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature