CRISPR-Analytics (CRISPR-A): A platform for precise analytics and simulations for gene editing.

ABSTRACT: Gene editing characterization with currently available tools does not always give precise relative proportions among the different types of gene edits present in an edited bulk of cells. We have developed CRISPR-Analytics, CRISPR-A, which is a comprehensive and versatile genome editing web application tool and a nextflow pipeline to give support to gene editing experimental design and analysis. CRISPR-A provides a robust gene editing analysis pipeline composed of data analysis tools and simulation. It achieves higher accuracy than current tools and expands the functionality. The analysis includes mock-based noise correction, spike-in calibrated amplification bias reduction, and advanced interactive graphics. This expanded robustness makes this tool ideal for analyzing highly sensitive case

SUBMITTER: Sanvicente-Garcia M 

PROVIDER: S-EPMC10256225 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature