Metabolic impact of genetic and chemical ADP/ATP carrier inhibition in renal proximal tubule epithelial cells.

ABSTRACT: Mitochondrial dysfunction is pivotal in drug-induced acute kidney injury (AKI), but the underlying mechanisms remain largely unknown. Transport proteins embedded in the mitochondrial inner membrane form a significant class of potential drug off-targets. So far, most transporter-drug interactions have been reported for the mitochondrial ADP/ATP carrier (AAC). Since it remains unknown to what extent AAC contributes to drug-induced mitochondrial dysfunction in AKI, we here aimed to better understand the functional role of AAC in the energy metabolism of human renal proximal tubular cells. To this end, CRISPR/Cas9 technology was applied to generate AAC3^-/- human conditionally immortalized renal proximal tubule epithelial cells. This AAC3^-/- cell model was characterized wi

SUBMITTER: Hoogstraten CA 

PROVIDER: S-EPMC10256673 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature