Leptin b and its regeneration enhancer illustrate the regenerative features of zebrafish hearts.

ABSTRACT:

Background

Zebrafish possess a remarkable regenerative capacity, which is mediated by the induction of various genes upon injury. Injury-dependent transcription is governed by the tissue regeneration enhancer elements (TREEs). Here, we utilized leptin b (lepb), an injury-specific factor, and its TREE to dissect heterogeneity of noncardiomyocytes (CMs) in regenerating hearts.

Results

Our single-cell RNA sequencing (scRNA-seq) analysis demonstrated that the endothelium/endocardium(EC) is activated to induce distinct subpopulations upon injury. We demonstrated that lepb can be utilized as a regeneration-specific marker to subset injury-activated ECs. lepb⁺ ECs robustly induce pro-regenerative factors, implicating lepb⁺ ECs as a signaling center to interact

SUBMITTER: Shin K 

PROVIDER: S-EPMC10256838 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature