Role of K364 next to the active site cysteine in voltage-dependent phosphatase activity of Ci-VSP.

ABSTRACT: Voltage-sensing phosphatase (VSP) consists of the voltage sensor domain (VSD) similar to that of voltage-gated ion channels and the cytoplasmic phosphatase region with remarkable similarity to the phosphatase and tensin homolog deleted on chromosome 10 (PTEN). Membrane depolarization activates VSD, leading to dephosphorylation of three species of phosphoinositides (phosphatidylinositol phosphates (PIPs)), PI(3,4,5)P₃, PI(4,5)P₂, and PI(3,4)P₂. VSP dephosphorylates 3- and 5-phosphate of PIPs, unlike PTEN, which shows rigid 3-phosphate specificity. In this study, a bioinformatics search showed that some mammals have VSP orthologs with amino acid diversity in the active center motif, Cx₅R, which is highly conserved among protein tyrosine phosphatase

SUBMITTER: Paixao IC 

PROVIDER: S-EPMC10257114 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature