Organotypic hippocampal culture model reveals differential responses to highly similar Zika virus isolates.

ABSTRACT:

Introduction

Zika virus (ZIKV) caused an outbreak in Brazil, in 2015, being associated to microcephaly. ZIKV has a strong neurotropism leading to death of infected cells in different brain regions, including the hippocampus, a major site for neurogenesis. The neuronal populations of the brain are affected differently by ZIKV from Asian and African ancestral lineages. However, it remains to be investigated whether subtle variations in the ZIKV genome can impact hippocampus infection dynamics and host response.

Objective

This study evaluated how two Brazilian ZIKV isolates, PE243 and SPH2015, that differ in two specific missense amino acid substitutions, one in the NS1 protein and the other in the NS4A protein, affect the hippocampal phenotype and transcriptome.

Methods

SUBMITTER: Oliveira MDS 

PROVIDER: S-EPMC10257278 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature