Ontology highlight
ABSTRACT:
SUBMITTER: Yarnall MTN
PROVIDER: S-EPMC10257351 | biostudies-literature | 2023 Apr
REPOSITORIES: biostudies-literature
Yarnall Matthew T N MTN Ioannidi Eleonora I EI Schmitt-Ulms Cian C Krajeski Rohan N RN Lim Justin J Villiger Lukas L Zhou Wenyuan W Jiang Kaiyi K Garushyants Sofya K SK Roberts Nathaniel N Zhang Liyang L Vakulskas Christopher A CA Walker John A JA Kadina Anastasia P AP Zepeda Adrianna E AE Holden Kevin K Ma Hong H Xie Jun J Gao Guangping G Foquet Lander L Bial Greg G Donnelly Sara K SK Miyata Yoshinari Y Radiloff Daniel R DR Henderson Jordana M JM Ujita Andrew A Abudayyeh Omar O OO Gootenberg Jonathan S JS
Nature biotechnology 20221124 4
Programmable genome integration of large, diverse DNA cargo without DNA repair of exposed DNA double-strand breaks remains an unsolved challenge in genome editing. We present programmable addition via site-specific targeting elements (PASTE), which uses a CRISPR-Cas9 nickase fused to both a reverse transcriptase and serine integrase for targeted genomic recruitment and integration of desired payloads. We demonstrate integration of sequences as large as ~36 kilobases at multiple genomic loci acro ...[more]