Single-cell protein profiling defines cell populations associated with triple-negative breast cancer aggressiveness.

ABSTRACT: Triple-negative breast cancer (TNBC) is an aggressive and complex subtype of breast cancer that lacks targeted therapy. TNBC manifests characteristic, extensive intratumoral heterogeneity that promotes disease progression and influences drug response. Single-cell techniques in combination with next-generation computation provide an unprecedented opportunity to identify molecular events with therapeutic potential. Here, we describe the generation of a comprehensive mass cytometry panel for multiparametric detection of 23 phenotypic markers and 13 signaling molecules. This single-cell proteomic approach allowed us to explore the landscape of TNBC heterogeneity, with particular emphasis on the tumor microenvironment. We prospectively profiled freshly resected tumors from 26 TNBC patients. The

SUBMITTER: Kvokackova B 

PROVIDER: S-EPMC10257414 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature